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Basic Stains 01

Topic: Pathology

Created on Saturday, February 28 2009 by jdmiles

Last modified on Saturday, February 28 2009.

Courtesy of Dr. Mark Cohen

The image above shows a slice of brain from a person who had encephalitis caused by Candida. The tissue is stained with periodic acid Schiff (PAS) stain. Which of the following does PAS stain for?

A) Lipid

B) Iron

C) Glycogen

D) Calcium

E) Collagen

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This question was created on February 28, 2009 by jdmiles.
This question was last modified on February 28, 2009.

ANSWERS AND EXPLANATIONS

A) Lipid

This answer is incorrect.

PAS highlights glycogen and basement membrane. Oil red O is a good stain for lipid (See References)


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B) Iron

This answer is incorrect.

PAS highlights glycogen and basement membrane. (See References)


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C) Glycogen

This answer is correct.

PAS highlights glycogen and basement membrane. (See References)


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D) Calcium

This answer is incorrect.

PAS highlights glycogen and basement membrane. Von Kossa is a good stain for calcium. (See References)


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E) Collagen

This answer is incorrect.

PAS highlights glycogen and basement membrane. Trichrome is an effective stain for collagen. (See References)


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References:

1. Prayson, R.A., and Goldblum, J.R. (Eds.) (2005). Neuropathology. Elsevier Churchill Livingstone, Philadelphia. (ISBN:0443066582)

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pathology
Basic Stains 01
Question ID: 022809094
Question written by J. Douglas Miles, (C) 2006-2009, all rights reserved.
Created: 02/28/2009
Modified: 02/28/2009
Estimated Permutations: 1200